J Bone Jt Infect 2018; 3(4):173-181. doi:10.7150/jbji.27840
Accuracy of Tissue and Sonication Fluid Sampling for the Diagnosis of Fracture-Related Infection: A Systematic Review and Critical Appraisal
1. Department of Trauma Surgery, University Hospitals Leuven, Belgium
2. Department of Laboratory Medicine, University Hospitals Leuven, Belgium
3. Department of Trauma Surgery, University Medical Center Utrecht, The Netherlands
4. Department of Infectious Diseases and Hospital Epidemiology, University Hospital of Basel, Switzerland
5. KU Leuven Libraries - 2Bergen - Learning Centre Désiré Collen, Leuven, Belgium
6. Department of Orthopaedic and Trauma Surgery, University Hospital Basel, Switzerland
7. The Bone Infection Unit, Nuffield Orthopaedic Centre, Oxford University Hospitals, Oxford, United Kingdom
8. Charité - Universitätsmedizin Berlin, corporate member of Freie Universität Berlin, Humboldt-Universität zu Berlin, and Berlin Institute of Health, Center for Musculoskeletal Surgery
Introduction: Intraoperatively obtained peri-implant tissue cultures remain the standard for diagnosis of fracture-related infection (FRI), although culture-negative cases may complicate treatment decisions. This paper reviews the evidence on sonication fluid and tissue sampling for the diagnosis of FRI.
Methods: A comprehensive search in Pubmed, Embase and Web-of-Science was carried out on April 5, 2018, to identify diagnostic validation studies regarding sonication fluid and tissue sampling for FRI.
Results: Out of 2624 studies, nine fulfilled the predefined inclusion criteria. Five studies focused on sonication fluid culture, two on PCR and two on histopathology. One additional histopathology study was found after screening of reference lists. There is limited evidence that sonication fluid culture may be a useful adjunct to conventional tissue culture, but no strong evidence that it is superior or can replace tissue culture. Regarding molecular techniques and histopathology the evidence is even less clear. Overall, studies had variable 'gold standard' criteria for comparison and poorly reported culture methods.
Conclusions: Scientific evidence on sonication fluid and tissue sampling, including culture, molecular techniques and histopathology for the diagnosis of FRI is scarce. It is imperative that laboratory protocols become standardized and uniform diagnostic criteria, as recently published in a consensus definition, be implemented.
Keywords: Fracture-related infection, histopathology, sonication, tissue sampling, diagnosis, systematic review
Onsea J, Depypere M, Govaert G, Kuehl R, Vandendriessche T, Morgenstern M, McNally M, Trampuz A, Metsemakers WJ. Accuracy of Tissue and Sonication Fluid Sampling for the Diagnosis of Fracture-Related Infection: A Systematic Review and Critical Appraisal. J Bone Jt Infect 2018; 3(4):173-181. doi:10.7150/jbji.27840. Available from http://www.jbji.net/v03p0173.htm